Peprotech Recombinant Human HGF（100-39）_详细资料

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Peprotech Recombinant Human HGF（100-39）北京杰辉是一家集销售生化试剂、实验耗材、自产分子生物学产品为一体，并能提供技术支持的专业性生物科技公司。公司秉承“以客户为中心，以产品为保障、以诚信为基础、以创新为宗旨”的发展理念，在依靠客户的大力支持和员工的不懈努力下获得了快速的成长。

Peprotech Recombinant Human HGF（100-39）
本试剂盒仅供研究使用。
使用目的：
本试剂盒用于测定血清、血浆及相关液体样本中待测物质的含量。
Peprotech Recombinant Human HGF（100-39） 实验原理
本试剂盒应用双抗体夹心法测定标本中待测物质水平。用纯化的待测物质抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入待测物质，再与HRP标记的待测物质抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成的黄色。颜色的深浅和样品中的待测物质呈正相关。用酶标仪在450nm波长下测定吸光度（OD 值），通过标准曲线计算样品中待测物质浓度。
Peprotech Recombinant Human HGF（100-39） 试剂盒组成
Peprotech Recombinant Human HGF（100-39） Catalog Number:
100-39
Synonyms:
Scatter Factor （SF）, Hepatopoietin （HPTA）
Description:
HGF is a mesenchymally derived potent mitogen for mature parenchymal hepatocyte cells ａnd acts as a growth factor for a broad spectrum of tissues ａnd cell types. HGF signals through a transmembrane tyrosine kinase receptor known as MET. Activities of HGF include induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, ａnd enhancement of neuron survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy ａnd other liver injuries. Human ａnd murine HGF are cross-reactive. Human HGF is expressed as a linear 697 amino acid polypeptide precursor glycoprotein. Proteolytic processing of this precursor generates the biologically active form of HGF, which consists of two polypeptide chains （α-chain ａnd β-chain） held by a single disulfide bond resulting in formation of a biologically active heterodimer. The α-chain consists of 463 amino acid residues ａnd four kringle domains. The β-chain consists of 234 amino acid residues.*Manufactured using （BTI-Tn-5B1-4） cells under license from Boyce Thompson Institute for Plant Research, Inc.
AA Sequence:
alpha chain: QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTADQCA NRCTRNKGLP FTCKAFVFDK ARKQCLWFPF NSMSSGVKKE FGHEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQP WSSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG LMDHTESGKI CQRWDHQTPH RHKFLPERYP DKGFDDNYCR NPDGQPRPWC YTLDPHTRWE YCAIKTCADN TMNDTDVPLE TTECIQGQGE GYRGTVNTIW NGIPCQRWDS QYPHEHDMTP ENFKCKDLRE NYCRNPDGSE SPWCFTTDPN IRVGYCSQIP NCDMSHGQDC YRGNGKNYMG NLSQTRSGLT CSMWDKNMED LHRHIFWEPD ASKLNENYCR NPDDDAHGPW CYTGNPLIPW DYCPISRCEG DTTPTIVNLD HPVISCAKTK QLR

beta chain: VVNGIP TRTNIGWMVS LRYRNKHICG GSLIKESWVL TARQCFPSRD LKDYEAWLGI HDVHGRGDEK CKQVLNVSQL VYGPEGSDLV LMKLARPAVL DDFVSTIDLP NYGCTIPEKT SCSVYGWGYT GLINYDGLLR VAHLYIMGNE KCSQHHRGKV TLNESEICAG AEKIGSGPCE GDYGGPLVCE QHKMRMVLGV IVPGRGCAIP NRPGIFVRVA YYAKWIHKII LTYKVPQS

Source:
（BTI‐Tn‐5B1‐4） Hi‐5 Insect cells*
Purity:
Greater than 98% by SDS-PAGE gel ａnd HPLC analyses.
Biological Activity:
The ED₅₀ was determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells was found to be in the range of 20.0-40.0 ng/ml.

Cross Reactivity:
Dog, Monkey, Mouse, Rabbit, Rat, Human + Mouse, Human, Leech

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