Product Description | This high affinity anti-HA antibody is developed using the HA peptide (YPYDVPDYA) conjugated to KLH and affinity-purified with the HA peptide. This antibody is conjugated to horse radish peroxidase (HRP) through reductive amination. Direct labelling of the primary antibody will avoid the use of secondary antibodies therefore eliminating the interference of the secondary antibody-conjugates. | | Western blot analysis of the HA-tag protein (50 kDa) expressed cell lysates with HA antibody HRP conjugates (ICP0751) 1: Marker 2: 25 µL/lane 3: 15 µL/lane 4: 5 µL/lane 5: 2 µL/lane A B C Western blot analysis of the positive cell lysates with expression of HA-tag protein. A: Marker B: 1 µL/lane C: 10 µL/lane |