AACT抗胰糜蛋白酶（兔多克隆抗体）

AACT抗胰糜蛋白酶（兔多克隆抗体）

α-1-Antichymotrypsin 

广州健仑生物科技有限公司

AACT是一种丝氨酸蛋白酶YZ剂，可以中和糜蛋白酶等酶活性的蛋白酶，存在于大多数的组织细胞、巨噬细胞以及多种胃肠道和肺部肿瘤中，但多形核白细胞中无此物质，它可以作为组织细胞瘤和良性/恶性纤维组织细胞瘤的标志。此抗体与人的AACT反应，主要用于恶性纤维组织细胞瘤等恶性肿瘤的诊断,也可用于胃癌、肺癌、肾癌等肿瘤的研究。

我司还提供其它进口或国产试剂盒：登革热、疟疾、流感、A链球菌、合胞病毒、腮病毒、乙脑、寨卡、黄热病、基孔肯雅热、克锥虫病、违禁品滥用、肺炎球菌、军团菌、化妆品检测、食品安全检测等试剂盒以及日本生研细菌分型诊断血清、德国SiFin诊断血清、丹麦SSI诊断血清等产品。

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【产品介绍】

细胞定位：细胞浆

适用组织：石蜡/冰冻

阳性对照：扁桃体

抗原修复：热修复（EDTA）

抗体孵育时间：30-60min

AACT抗胰糜蛋白酶（兔多克隆抗体）

刚开始尝试“培养”视网膜时，我们实验室还在探讨视网膜形成的一些基本问题。我们知道，视网膜是从胎儿大脑中名为“间脑”(diencephalon)的那一部分发育而来的。在胚胎发育的早期阶段，间脑的一部分会扩展，形成气球状的视泡(optic vesicle)，后者再向内凹陷，形成视杯;视杯进一步形变，Z终成为视网膜。
一个多世纪以来，生物学家一直就视杯形成的精确机制争论不休，直到今天，研究大脑发育的科学家仍然各执一词。其中一个较有争议的问题是，在视杯形成过程中，与之相邻的一些结构，如晶状体和角膜起了什么作用?有些科学家认为，视网膜向内凹陷，是因为受到了晶状体的物理推动作用;也有科学家认为，视杯无须借助晶状体的作用，就可以自己形成。
要想在活着的、正在发育中的动物身上观察这一现象绝非易事，因此大约在10年前，我的研究团队决定做一次尝试，看能不能把眼睛的发育过程“提取”出来。
具体做法是，先在培养皿中培养胚胎干细胞，然后加入眼睛发育所需的化学物质，观察培养皿中发生的情况。从发育程度上来说，胚胎干细胞是Z原始的干细胞，Z终可以分化成从神经到肌肉的各种组织。
当时，把干细胞培育成器官的技术尚不存在。人们曾把相互分离的干细胞“撒”在膀胱或食管形状的人工骨架上，试图搭建出新的器官。这类组织工程学技术在培植真实器面并不是很成功。
因此，我们决定另辟蹊径。在正式动手之前，我们做了一些准备工作。2000年，我们发明了一种细胞培养方法，可以把小鼠的胚胎干细胞转变成多种神经细胞。随后，我们在培养皿中培养了一层小鼠胚胎干细胞，并加入一些可充当“传递员”的细胞——这些细胞会向胚胎干细胞传递化学信号，促使后者发育、分化，脱离胚胎状态。我们培养这些细胞的目的，并不是要复制某个人体器官的三维结构，而是想看看，仅用细胞自身的化学信号，是否足以让胚胎干细胞形成眼睛发育早期所独有的神经细胞。
起初，我们没有获得多大的成功，但在2005年，我们在技术上取得了突破。以前，我们实验室在培养干细胞时，细胞只能平铺在培养皿上，但在2005年，我们突破了“二维限制”，可以让干细胞悬浮在培养液中，这就是“悬浮培养”。我们采用这种三维培养技术的原因有很多。首先，在悬浮培养中，细胞聚集时，本身就会形成三维结构，因此在产生复杂组织时，会比平铺的细胞层更容易;其次，为了发育成复杂的结构，细胞之间需要相互交流，而三维培养更适于促进这样的交流，因为细胞之间可以更加灵活地发生相互作用。
使用这种新方法，我们把相互分离的胚胎干细胞悬浮在液体培养基中，然后注入多孔培养皿的小孔中(每个小孔只有微量的培养基，含大约3 000个胚胎干细胞)。我们发现，原本分开的胚胎干细胞开始聚集在一起。

我司还提供其它进口或国产试剂盒：登革热、疟疾、流感、A链球菌、合胞病毒、腮病毒、乙脑、寨卡、黄热病、基孔肯雅热、克锥虫病、违禁品滥用、肺炎球菌、军团菌、化妆品检测、食品安全检测等试剂盒以及日本生研细菌分型诊断血清、德国SiFin诊断血清、丹麦SSI诊断血清等产品。

想了解更多的产品及服务请扫描下方二维码：

【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

At the beginning of trying to "cultivate" the retina, our laboratory is still exploring some of the basic problems of retinal formation. We know that the retina develops from that part of the fetal brain called diencephalon. In the early stages of embryonic development, a portion of the diencephalon expands to form a balloon-shaped optic vesicle, which in turn sinks inwardly to form an optic cup; the optic cup further deforms to eventually become the retina.
For more than a century, biologists have been arguing for the exact mechanism by which cups are formed. Until today, scientists studying brain development remained silent. One of the more controversial issues is the role of adjacent structures such as the lens and cornea during optic cup formation. Some scientists believe that the retina is inwardly depressed because of the physical impetus of the lens Role; also some scientists believe that the cup without the help of the role of lens, you can form their own.
Observing this phenomenon on living, developing animals is by no means an easy task, so about 10 years ago my team decided to make an attempt to "extract" the development of the eye.
This is done by first culturing embryonic stem cells in a petri dish and then adding the chemicals needed for eye development to observe what is happening in the petri dish. In terms of development, embryonic stem cells are the most primitive stem cells that eventually differentiate into various tissues ranging from nerve to muscle.
At the time, the technology to grow stem cells into organs did not exist yet. People have "sprinkled" separated stem cells on the artificial skeleton of the bladder or esophagus in an effort to build new organs. Such tissue engineering techniques are not very successful in developing real organs.
Therefore, we decided to find another way. Before we started, we made some preparations. In 2000, we invented a cell culture method that transforms mouse embryonic stem cells into a variety of nerve cells. We then cultured a layer of mouse embryonic stem cells in a Petri dish and added cells that act as "transferees" - these cells send chemical signals to the embryonic stem cells that cause the latter to develop, differentiate, and detach themselves from the embryo. Instead of trying to copy the three-dimensional structure of a human organ, we want to see if the chemical signals of the cells alone are enough for embryonic stem cells to become neurons that are unique to early eye development.
At first, we did not get much success, but in 2005, we made a technological breakthrough. In the past, when we cultured stem cells in our laboratory, the cells were only laid on the culture dish. However, in 2005, we broke through the "two-dimensional limitation" and allowed the suspension of stem cells in the culture medium. This is called "suspension culture." There are many reasons why we use this three-dimensional culture technique. First, in suspension culture, cells accumulate and form three-dimensional structures themselves, making them easier to produce complex tissues than tiled cell layers. Second, cells need to communicate with each other in order to develop complex structures , While three-dimensional cultivation is better suited to facilitate such exchanges because the cells can interact more flexibly.
Using this new method, we suspended the embryonic stem cells isolated from each other in liquid medium and injected them into the wells of a multi-well culture dish (with only a minimal amount of media per well containing approximay 3,000 embryonic stem cells). We found that originally separated embryonic stem cells began to congregate.