G030 G418 disulfate, EvoPure/G418硫酸氢盐, EvoPure

G418 disulfate, EvoPure/G418硫酸氢盐, EvoPure
产品编号：G030 
产品规格：1g/5g/10x5g

CAS Number	108321-42-2
Molecular Formula	C20H40N4O10 · 2H2SO4
Molecular Weight	692.71 g/mol
Form	Powder
Purity Level	≥99.0%
Solubility	Soluble
Source	Biosynthetic: produced by Micromonospora rhodorangea.
Boiling Point	1012.1 °C
Flash Point	565.9 °C
Storage Conditions	2-8 °C

Description
G418 disulfate also known as geneticin, is an aminoglycoside antibiotic commonly used in plant and mammalian cell culture as a selectable marker. Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein.
TOKU-E offers three forms of G418: G418 disulfate (G001), G418 disulfate, EvoPure (G030), and G418 disulfate solution (G020). G418 disulfate and G418 disulfate, EvoPure? are soluble in aqueous solution. G418 disulfate solution is G418 dissolved in water at 50 mg/mL.  EvoPure? products are purified single antibiotic fractions, most >99% pure.
Mechanism
G418 blocks polypeptide synthesis in eukaryotic cells by binding irreversibly to 80S ribosomes and therefore disrupting their proofreading capability.
Plant Biology Applications
Dong and McHughen (1992) compared the use of kanamycin and geneticin as a selection agent in the genetic transformation of flax by Agrobacterium mediated transformation. Experiments in sweet potato showed G418 to be a more reliable selection agent with a higher transformation efficiency compared to kanamycin.
Mammalian Biology Applications