HAC-84细胞,人肺癌细胞冷藏ZX 货号:GD-C18919250
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Methyl 4-hydroxy-3-nitrobenzoate, 98+%
6-Hydroxy-1-indanone
4-BENZYLOXY-6-CHLOROPYRIMIDINE
2-Chloro-4-(trifluoroMethyl)phenyl isocyanate
Furan-2,5-diboronic acid, pinacol ester
L(-)-Borneol
2-Methyl-4-cyanophenylboronic acid
alpha-Methylcinnamic acid, 99%
2-Hydroxymethyl-4-methoxypropoxy-3-methylpyridine ,97%
2-Chloro-3-(trifluoromethyl)phenylboronic acid
N,N-Dimethylselenourea, 97%
6-Methoxynicotinaldehyde
N-AMYL BENZOATE
2-CHLORO-4,6-DIBROMOANILINE
Ethyl-3-cyclopropyl-3-oxopropionate
2,6-DibroMo-4-(trifluoroMethyl)aniline
Dichloro(1,10-phenanthroline)copper(II)
Methyl 5-broMo-2-hydroxy-3-Methoxybenzoate
Zinc stearate
SUCCINIC ACID
4-HydroxyphenyldiMethylsulfoniuM Methyl Sulfate
2,4-Dinitrophenylacetic acid
2-acetamido-5-hydroxy-7-naphthalenesulfonic acid
2-Nitrophenyl disulfide
4-Fluorocinnamic acid
2-Methylbenzyl cyanide
ETHYL 2-(BROMOMETHYL)ACRYLATE
PhosphoMolybdic Acid Hydrate
2-AMinothiophenol, 98%
2-AMino-4,5,6,7-tetrahydrobenzo[b]thiophene-3-carboxaMide, 96%
2,4,5-T butoxyethyl ester
LithiuM iodide hydrate
MAGNESIUM ACRYLATE
OCTANOPHENONE
3-BroMo-5-fluoro-2-isopropoxyphenylboronic acid
METHYL 3-CHLORO-4-METHOXYBENZOATE
N1-(3-Acetyl-4-hydroxyphenyl)acetamide, 97%
Tridecafluorohexyl Bromide
1,4-Bis(mercaptomethyl)benzene
Yttrium(III) 2-ethylhexanoate (15-17% Y), superconductor grade (99.9%-Y) (REO)
2-METHYL-3-FUROIC ACID
1,2-Bis(bromomethyl)benzene
4-Chloro-D-phenylalanine methyl ester hydrochloride
L-Serine methyl ester hydrochloride
3-ChroManecarboxylic acid
2-AMino-5-chloro-2′-fluorobenzophenone
Ethyl Heptadecafluorononanoate
4-Chloro-7-trifluoroMethylquinoline
N-Methyl-N-(2-thien-2-ylbenzyl)aMine, 97%
OXALYL BROMIDE
2',4',5',7'-TetrabroMo-3,4,5,6-tetrachlorofluorescein
1,5-dichloro-1,1,3,3,5,5-hexamethyltrisiloxane
2-Amino-4-methyl-5-bromopyrimidine
4-ethylbenzaldehyde
1-(benzyloxy)-2-methoxy-4-((Z)-2-nitroethenyl)benzene
4-N-PROPOXYBROMOBENZENE
Iodoacetonitrile
3-Chloro-2-fluoro-5-(trifluoroMethyl)pyridine
N-Isopropyl 3-broMo-5-nitrobenzaMide
2-Chloro-2',4'-difluoroacetanilide 97%
2-Iodobenzenesulphonic acid
2,3-Bis(methylthio)-1,4-naphthalenedione
4-(tert-Butyl)-3-methoxybenzoic acid
DESOXYANISOIN
2-CHLORO-4-FLUOROBENZENESULFONYL CHLORIDE
2-Amino-6-chlorobenzotrifluoride
2-Methoxynaphthalene
1-(2-Furoyl)
1-BroMo-4-dodecylbenzene
3,5-DiMethyl-1h-pyrrole-2,4-dicarboxylic acid 2-tert-butyl ester 4-ethyl ester
AMMoniuM Molybdate, 99.99%, (trace Metal basis)
D-1,2,3,4-Tetrahydroisoquinoline-3-carboxylic acid, 98%
(4-Chloro-2-Methylphenoxy)acetic Acid
N-(tert-Butoxycarbonyl)-L-isoleucine HeMihydrate
Di-n-butyl sulphate
1H-Pyrazolo[3,4-b]pyridine-3-carboximidic acid,1-[(2-fluorophenyl)methyl]-, methyl ester
4-[3-ethoxy-2-(hydroxyl) propoxy]aniline
Propanoicacid,3-(cyclohexylthio)-,ethylester
CFL2 ELISA Kit
Mouse Soluble protein-100,S-100 ELISA Kit
Human PCDHβ14 ELISA Kit
Human FDFT1 ELISA Kit
TSubset ELISA Kit
β2M ELISA Kit
EMA IgA ELISA Kit
Human CCK-8 (Cholecystokinin 8) ELISA Kit
Human HA ELISA Kit
AChE ELISA Kit
Aeromonas Pyrazinamidase activity Medium
Modified Cellobiose-Polymyxin B-Colistin Agar Base
Diethyl acetylMalonate, tech. 85%
AMMoniuM Hydrogen Fluoride
5,5-DibroMo-2,2-Bithiophene
2-Fluoro-4-(trans-4-pentylcyclohexyl)-4'-(trans-4-propylcyclohexyl)biphenyl, 97%
2,4-Dinitrophenylacetic acid
Z-D-Orn(Z)-OH
Cedrene
Azetidine
1-Methylcyclohexanol
5-MERCAPTO-(1H)-TETRAZOLYLACETIC ACID SODIUM SALT
PyriMidine-2-Carboxylic Acid
IndiuM antiMonide, Electronic Grade, 99.99% (Metals basis)
3-AMino-4-ethyl-1H-pyrazole, 98%
Benzyl 2-azabicyclo[3.3.0]octane-3-carboxylate hydrochloride
AntiMony acetate
3-N-PROPYL-2-PYRAZOLIN-5-ONE
2-Chlorocyclohexanone
Uridine-5'-triphosphoric acid trisodiuM salt
Boc-Nalpha-Methyl-D-alanine
2-(4-Isocyanatophenyl)pyrimidine, 90%
7-Chloro-1H-indole-3-carboxylic acid
Methyl (methylthio)acetate
4'-Chloro-3'-hydroxyacetanilide