NCI-H446[H446]细胞,人肺癌细胞冷藏ZX 货号:GD-C18919148
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2,4,5-Trifluorobenzonitrile, 98+%
3,5-Difluorophenylboronic acid
Diethylene glycol diacrylate
BenzeneMethanaMine, 3-(difluoroMethoxy)- (9CI)
BIS(PENTAMETHYLCYCLOPENTADIENYL)OSMIUM
LITHIUM TUNGSTATE
Trans-2-(4-Methylphenyl)vinylboronic acid
4-n-Butylbenzenesulfonyl chloride
5-Fluoro-2-methylbenzoic acid
Iodipamide
2-Iodo-1-trityl-1H-imidazole
1,1,1-TRICHLOROTRIFLUOROACETONE
FMOC-L-2-Fluorophe
FMOC-(S)-3-AMINO-3-(4-BROMO-PHENYL)-PROPIONIC ACID
2,1,3-Benzothiadiazole-4-sulfonyl chloride
2-EthylhexylaMine
1,2-DiMethylbenziMidazole
Valeroin
Tris(pentafluorophenyl)phosphine, 97%
SodiuM Acetate, Anhydrous, USP
2-Fluoro-4-nitrobenzoic Acid
4-(2-Furyl)-3-buten-2-one
Beta-Naphthoflavone
3-acetyl-1-propanol
3-Fluoro-4-nitrophenol
N,N'-DIALLYL-L-TARTARDIAMIDE
L(+)-Ascorbic acid
2-AMino-5-Ethylphenol Hydrochloride
3-Acetylbenzo[b]thiophene, 98%
1-(2-Methoxyphenyl), 98%
3-AMino-4-broMo-1H-indazole, 97%
Tetrafluoro-2-(tetrafluoro-2-iodoethoxy)ethanesulfonyl fluoride
Boc-D-Cha-OH
Phenyl chloroforMate
Methyl 2-aMino-4,5-diMethoxybenzoate
4,4'-DIMETHYLBIPHENYL
4-PiperidineMethanol
Tris(cyclopentadienyl)praseodymium (99.9%-Pr) (REO)
Pepstatin A
6-Methoxy-3,4-dihydro-1H-quinolin-2-one 98%
Boc-(2R,3R)-3-amino-2-hydroxy-5-methylhexanoic acid
3-FLUORO-5-(TRIFLUOROMETHYL)BENZYL BROMIDE
MAGNESIUM ACETYLACETONATE
5-Bromosalicylic acid
2-Chloro-5-methylpyridine
2,4-DiMethyliMidazole
2,4'-Difluorobenzophenone
4-BroMo-2-MethyliMidazole
PotassiuM tetrafluoroborate, 99%, extra pure
Glycyrrhizic Acid MonoaMMoniuM Salt, Trihydrate
2-Butanol
Perylene
1-(methylsulphinyl)dodecane
norfenefrine
DL-Terebic acid
2,3,4,6-TETRAFLUOROPYRIDINE
(R)-(S)-JOSIPHOS
4-FluorophenethylaMine
2-BroMovaleric acid
2-[2-(Diethylamino)ethoxy]benzenecarbaldehyde
4-Methoxyphenyl isothiocyanate, 97%
Sodium phosphate, tribasic, anhydrous, pure
Ethyl 2-Hexylacetoacetate
(2R,3S)-3-Phenylisoserine
Cupric acetate monohydrate
Fenofibric acid
Boron trifluoride dimethyl etherate
Ethyl forMate
1-AMinopentadecane
PiperidiniuM acetate
3-Chlorophenol, 99%
Nitrofurantoin, 98%
L-Ornithine L-aspartate salt
trans-Decahydroquinoline
p-Cymene
[(S)-(-)-2,2'-Bis(diphenylphosphino)-1,1'-binaphthyl]palladium (II) Chloride
Disperse Red 179
Azure II
HAase ELISA Kit
ULBP2 ELISA Kit
Pre-APO-C2 ELISA Kit
Human DEFβ110 ELISA Kit
Chiken Pullorum Diseaes,PD ELISA Kit
WFS1 ELISA Kit
FAS ELISA Kit
Human MAOA (Type A Monoamine Oxidase)ELISA Kit
Human ANGPTL6 ELISA Kit
Fbg ELISA Kit
Sterile Soybean-Casein Digest Agar Medium
Medium 5(Streptomycin Assay Agar)
Copper(II) isopropoxide, 98% (Metals basis)
Diethyl FuMarate
5-Chloro-1-MethyliMidazole, 98%
Methyl 2-Fluoro-5-Nitrobenzoate
4-(2-Furyl)-3-buten-2-one
Di-t-butoxydiacetoxysilane
2-[2-(DIPHENYLPHOSPHINO)ETHYL]PYRIDINE
Alpha,Alpha,Alpha-Trifluoro-p-tolunitrile
1-Chlorooctane
TERT-BUTYL N-(BENZYLOXY)CARBAMATE
Xanthone
Bis(1,5-cyclooctadiene)rhodiuM(I) hexafluoroantiMonate
PotassiuM 4-tert-butylphenyltrifluoroborate, 96%
Ethylcyclohexane
Cobalt chloride hexahydrate
Diphenic acid
(1-OCTYL)TRIPHENYLPHOSPHONIUM BROMIDE
Pyrido[2,3-b]pyrazine
2,5-DIPHENYLTHIOPHENE
1,4-Cyclohexanedione ,98%
Glycolic Acid
1-Butyl-3-methylpyridinium Bromide
2-Chloro-5-methoxyphenol 98%