A-427细胞,人肺癌细胞冷藏ZX 货号：GD-C18919243
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
3,5-Dinitrobenzoyl chloride, 98+%
Cyphenothrin
4-ETHOXYCINNAMIC ACID
1,2-DIIODOETHANE
5-BroMo-2-cyanopyriMidine
BAPTA tetraethyl ester, 98+%
VeMurafenib
6-Isothiocyanato-2,3-dihydro-1,4-benzodioxine
alpha-Toluenethiol, 99%
4-n-Octyloxyphenyl 4-Butylbenzoate [Liquid Crystal]
N,N-Didodecylmethylamine
5-Nitroisatin
Divinyl sulphone
2,4-Dichlorobenzoyl chloride
3-Mercapto-1,2-propanediol
4,5-Dihydro-1,3-benzodioxine-6-aMine
2,5-Di(2-thienyl)-1H-pyrrole
2-BroMo-4-chlorobenzaldehyde
Zinc cyanide, 98%, pure
StearylaMine
2,2'-Oxybis(ethylaMine)
3,4,5-Trimethoxyphenol
Decahydroisoquinoline
N-Benzyloxycarbonyl-L-valine
3-Fluorocinnamic acid
Rhodium(II) acetate dimer
2-Diazo-1-naphthol-4-sulfonate
2-Ethylhexyl acrylate, 98%
5-(4-Fluorophenyl)valeric acid, 97%
3-[Methoxy(Methyl)carbaMoyl]benzeneboronic acid, 97%
Spirodiclofen
4,4-DIFLUORO-L-PROLINAMIDE HYDROCHLORIDE
2,2′-BiphenyldiaMine
OleaMide
NICKEL TELLURIDE
3-(Piperidine-1-carbonyl)phenylboronic acid
2,3-Dihydroxy-6-chloroquinoxaline ,97%
4-Cyclohexylbenzoic Acid
3-N-Cbz-Amino-piperidine
Thiochroman-4-one, 98%
3-Amino-4-chloropyridine
Carbobenzoxyhydrazide
3-(Bromomethyl)biphenyl
2-Chlorobenzamide
6-Chlorobenzo[d]isoxazol-3-ol
N-(tert-Butoxycarbonyl)-p-toluenesulfonaMide
4-Carboxy-2-fluorophenylboronic acid, pinacol ester
Anhydrotetracycline hydrochloride, 'can be used as a secondary standard'
3-Methoxy-2-nitropyridine, 98%
4-AMinostilbene
2-Cyanoethyltriethoxysilane
Isopropyl formate
Pyrazolo[1,5-a]pyridine-3-carboxylicacid, 4,5,6,7-tetrahydro-
3-Bromocinnamaldehyde
ethyl 5-oxo-DL-prolinate
1,3-Dichloro-5,5-dimethylhydantoin
D-Histidine
2-(4-Chlorobenzylthio)ethanol, 98%
(3-CYANOPROPYL)DIMETHYLCHLOROSILANE
2,4-Dichloro-3-ethyl-6-nitrophenol
Iron(II) chloride, anhydrous, 99%
4-Benzyloxy-1H-indole-3-carboxaldehyde, N-BOC protected 98%
1,3-Bis(1-adamantyl)imidazolium chloride, min. 97%
2,4-Dimethylaniline-6-sulfonic acid
4,5,6,7-Tetrahydrothieno[3,2,c] pyridine hydrochloride
(S)-(-)-2-T-BUTYL-2-CARBOXAMIDE
Ethylcyclohexane;
5-BroMobenzo[b]thiophene-3-carbaldehyde
1-BroMo-4-(phenylethynyl)benzene
3-Fluoro-4-(trifluoroMethoxy)phenylboronic acid
Di-n-octyltin oxide
[1,2,4]Triazolo[4,3-a]pyridine-3-thiol, 97%
Oil Orange SS
cis-1,3-CyclohexanediaMine
novobiocin sodium salt
(3R)-3-Amino-3-(4-bromophenyl)propan-1-ol
1-phenyl-3-(trifluoromethyl)pyrazole-4-carboxylic acid
5-bromoisoquinolin-8-amine
RLN ELISA Kit
Mouse S100A12 (S100 Calcium Binding Protein A12) ELISA Kit
Human PCDHβ5 ELISA Kit
Human TPT1 ELISA Kit
IFN-α ELISA Kit
Rat β2-glycoprotein 1 antibody IgA/G/M,β2-GP1 IgA/G/M ELISA Kit
Chicken Alternative macrophage activation-associated CC chemokine 1,AmAC-1 ELISA Kit
Human cholecystokinin octapeptide,CCK-8 ELISA Kit
Human Plasma fibronectin,pFN ELISA Kit
ADH1 ELISA Kit
Aeromonas Medium Base （Rayn）
Wagstsuma Blood Agar Base
ACETYL PHOSPHATE LITHIUM POTASSIUM SALT
SodiuM Hydrogen Difluoride
5,6,7,8-Tetrahydro-2-naphthol
2-Fluoropyridine-4-Boronic Acid
3,4,5-Trimethoxyphenol
1-Propargyl glycerol ether
Methylhesperidine
4-Ketopimelic acid
1,4-Cyclohexanebis(methylamine)
L(+)-2-Aminobutyric acid
2-Chloro-N-(2-fluorobenzyl)aniline, 97%
1,2-Bis(diMethylphosphino)ethane, 97%
2-Chloro-N-(2-fluorobenzyl)benzaMide, 97%
Diphenylvinylphosphine
4,4'-Stilbenedicarboxylic acid
DL-β-AMinoisobutyric acid
(Acetonitrile)[(2-biphenyl)di-tert-butylphosphine]gold(I) hexafluoroantiMonate
2-Hydroxy-4-Methyl-3-nitropyridine
Boron tribroMide
Sodium tert-Butoxide
2-Chloro-6-phenylnicotinonitrile
3-Iodophenylacetonitrile
2-Hydroxy-6-(trifluoromethyl)nicotinic acid
