NCI-H1688细胞,人肺癌细胞冷藏ZX 货号:GD-C18919104
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2-Ethylbutyraldehyde, 98%
Silver Metavanadate
3-tert-Butyloxy-1-propanol
Z-D-β-Dab(FMoc)-OH
cis-9-Octadecene-1-thiol
2,3-Pyridinedicarboxylic anhydride
4-Nitrophenyl Hexanoate , 98%
4-chloro-3-(2,5-dimethyl-1H-pyrrol-1-yl)benzoic acid(SALTDATA: FREE)
3-(2,6-Dichlorophenyl)propionic acid
Amylin, human
Rhodamine B octadecyl ester perchlorate
(R)-(+)-1,1'-Bi-2-naphthol
2,4,4'-TRIHYDROXYBENZOPHENONE
OCTAFLUORO-2-BUTENE
N,N,N',N'-TetraMethyl-p-phenylenediaMine dihydrochloride
2,6-DiMethylbenzoic acid
2',4'-Difluoroacetanilide
Methyl 5-broMo-2-chlorobenzoate
Trioctylphosphine, 90%, technical grade, AcroSeal
RhodaMine B
2,5-Dihydroxy-1,4-benzoquinone
Tri-n-propyl orthoformate
4-Methylbenzeneboronic acid
6-Methoxy-1,3-benzothiazole-2-carboxylic acid
2,6-Dihydroxybenzaldehyde
Methyl 4-hydroxyphenylacetate
MAGNESIUM CHLORIDE
Cobalt(II) sulfate heptahydrate
4-(Methylthio)phenylacetic acid, 97%
VanadiuM(V) triisopropoxide oxide, 96%
3-Methyl-1-butanol
Thiurea dioxide
2,4-DIMETHYL-3-PENTANOL
PHOSPHOROUS ACID TRIS(2-ETHYLHEXYL) ESTER
1,4-DICHLORO-2-IODOBENZENE
Propen-1-ylboronic acid, pinacol ester
4-Aminobutyric acid, 99+%
Bromomethyl methyl ether
N-Boc-(3'-Chlorophenyl)glycine
4-Acetamidophenylglyoxal hydrate 95%
2-Bromo-4-nitroimidazole
Ambroxane
ETHYL 3,5-DINITROBENZOATE
2-Methyl-2-butene;TriMethylethylene;β-IsoaMylene;AMylene
2,3-Dihydro-1,4-benzodioxin-5-ylMethylaMine hydrochloride
EriochroMe blue SE
Octadecyl Vinyl Ether
1-[3-(ChloroMethyl)-4-Methoxyphenyl]ethan-1-one
(4-BroMophenyl)cyclopropylMethanone, 95%
PHTHALOCYANINE
2,4,6-Trichloroaniline
Morroniside
2,3-Dimethoxypyridine
Desmopressin Acetate
ronidazole
4-Chlorovalerophenone
1,10-DIACETOXYDECANE
N-Ethylaniline
4-Fluoro-1-indanone
Undecafluorohexanoic Acid
1-(3,4-Dimethoxyphenyl) hydrochloride 97%
1-(5-Bromopyrimidin-2-yl)piperidine-3-carboxylic acid
2-Cyano-5-fluorophenylboronic acid pinacol ester
2,6-Dimethoxybenzoic acid
(2-BROMO-PYRIDIN-4-YL)CARBAMIC ACID TERT-BUTYL ESTER
1,3-Nonanediol acetate
N,N-Dimethyltrimethylsilylamine
CeriuM(IV) sulfate tetrahydrate
1-Acetoxy-4-diethylaMino-2-butyne
4,4'-(2-Hydroxybenzylidene)bis(2,3,6-triMethylphenol)
(2S,4R)-Boc-4-phenyl-pyrrolidine-2-carboxylic acid
4-(1H-IMidazol-1-yl)benzaldehyde, 97%
4-Amino-1-benzylpiperidine
2-BroMo-5-fluorotoluene
5-chloro-1,10-phenanthroline
Methyl-(N,N-DiethylaMinoMethyl)diethoxysilane
4-acryloylmorpholine
3-Methylphenethyl alcohol
VD ELISA Kit
α1-MG ELISA Kit
Human PS1 (Presenilin 1) ELISA Kit
Human PTPN6 ELISA Kit
FGF9 ELISA Kit
TLR2 ELISA Kit
C4 ELISA Kit
Human Surv ELISA Kit
VCAM-1 ELISA Kit
VEGF ELISA Kit
White Medium
Glfu Anaerobic Medium Agar Base,Modified
BariuM isopropoxide, 20% w/v in isopropanol
H-GLY-PNA
5-Chloro-2-Methylindole, 98%
2-Fluorobenzyl Chloride
Tri-n-propyl orthoformate
Solvent Blue 67
Acrylic acid Polymers
5-Chloro-3-fluorosalicylaldehyde
2,3,5,6-Tetrafluoroaniline
ETHYL PHENYLPHOSPHINATE
BenzyltriMethylaMMoniuM BroMide
1-Benzyl-1,4,8,11-tetraazacyclotetradecane
2-CyclohexylethylaMine, 97%
N,N-DiMethylacetaMide-d9
Lead telluride
2-OXOPENTANOIC ACID
alpha,alpha-Diphenyl-4-piperidinoMethanol
MARGAROYL CHLORIDE
1-Benzyloxy-2-Methoxy-4-propenylbenzene
1H,1H,5H-Octafluoropentyl acrylate
2',4'-Dimethoxyacetophenone
2-Amino-5-chloro-3-nitropyridine
4-Formylcinnamic acid, predominantly trans, 98%