293 Ad5细胞系——人原胚肾转化细胞系消化注意事项 货号:GD-C11519231
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
GerManiuM(IV) Methoxide, 98%
1,5-Dichloropentane
BUTYL-2-METHYLVALERATE
1,2,3-CYCLOHEXANETRIOL
Nonanoyl chloride, 97%
N-Cyclopropyl 4-(4-broMopyrazol-1-yl)benzenesulfonaMide
Methyl 4-aMino-3-Methylbenzoate
2-Fluoro-3-nitrobenzotrifluoride
4-Bromo-3-methylthiophenecarboxylic acid
4-(3-Methoxy-4-nitrophenyl)morpholine
5(6)-Carboxy-2',7'-dichlorofluorescein
3-Methylphenyl-D-alanine
1,1,1-Trifluoro-2,4-pentanedione
PLATINUM(IV) IODIDE
D(+)-Carvone
DiMethyl aMinoterephthalate
1-[2-(Di-tert-butylphosphino)phenyl]-3,5-diphenyl-1H-pyrazole
1-BOC-4,4-(ethylenedioxy)piperidine
YtterbiuM, 99.9%, (trace Metal basis), -12 MM
SpearMint Oil, FCC
(1R,2R)-2-(Naphthalene-2,3-dicarboxiMido)cyclohexanecarboxylic Acid
fluoroacetamide
2-Amino-6-hydroxy-8-mercaptopurine
Z-GLY-GLY-OET
3-chlorobenzenediazonium tetrafluoroborate
PHENYL FORMATE
ISOBUTYLAMINE HYDROCHLORIDE
TetraphenylphosphoniuM BroMide
2'-Fluoro-5'-(trifluoroMethyl)acetophenone, 97+%
Poly(Methyl-3,3,3-trifluoropropylsiloxane), M.W. 14,000
Luteolin
1,6-Hexanediol diMethacrylate
2,5-DIAMINOTOLUENE DIHYDROCHLORIDE
Oxacyclotridecan-2-one
FMoc-L-2-indanylglycine
4'-PIPERIDINOACETOPHENONE
Hexamethylphosphortriamide-D18 >99.5 Atom % D
3-Amino-2-bromobenzotrifluoride 98%
Triphenyl phosphate, 99+%
Basic Blue 1
3,3-Dimethylbutyric acid
Metronidazole
2-Chloroaniline
Epiandrosterone
2-Chloro-5-nitrobenzaMide
Tris-(8-hydroxyquinoline)aluMinuM
Triethoxy(3-glycidyloxypropyl)silane
2-Methyltetrahydrofuran, 99+%, Extra Dry, stabilized, AcroSeal
2-AMino-5-Methylbenzoic acid, 97%
1,3-Propanediol
4-Octadecyloxybiphenyl
Sodium nicotinate
[(2-furanylmethyl)sulfinyl]Acetic acid
2-Methylcyclohexanol acetate
pirenzepine dihydrochloride
3-Fluorophenylacetic acid
4-CHLOROPHENYLGLYOXYLOHYDROXAMYL CHLORIDE
3-Isobutoxy-4-Methoxyphenylboronic acid
TetrabutylaMMoniuM p-Nitrophenoxide
6-Methyl-3H-pyrimidin-4-one
4-Isopropylphenol, 98%
N,N-Dimethyl-4-fluoro-2-nitroaniline
6-Phosphogluconate Barium Salt Hydrate
2,2,2-TRIFLUOROETHYL TRICHLOROMETHANESULFONATE
1,1,1-TRIFLUOROPROPANE
Tetrabutylammonium hexafluorophosphate
Creatine monohydrate
2,6-Difluoro-3-nitrobenzonitrile
1-BroMo-3,5-diMethyladaMantane
Tridecyl Methacrylate (Mixture of branched chain isoMers) (stabilized with MEHQ)
3-Chloro-6-cyclopropylaMinopyridazine
2,4-Difluoronitrobenzene, 99%
DODECYL ACETATE
4,4,4-Trifluoro-1-(2-naphthyl)-1,3-butanedione
Nickel chloride
p-Hydrazinobenzoic acid hydrochloride
Sodium Glucuronate
beta-Alanine methyl ester hydrochloride
MPO ELISA Kit
S100A9 ELISA Kit
Human TMOD3 ELISA Kit
Human UBQLN3 ELISA Kit
IFN-β ELISA Kit
β4GALT1 ELISA Kit
bFGF ELISA Kit
CCK ELISA Kit
Human GPX3 ELISA Kit
GC ELISA Kit
Modified Cellobiose-Polymyxin B-Colistin Agar Base
Saline Lactose Broth Medium
Acetoxyacetyl Chloride
TitaniuM(IV) fluoride, 98%
5,6-Dichloronicotinic Acid
2-Fluoro-4-Methyl-5-Nitro Pyridine
fluoroacetamide
Borane-N,N-diethylaniline complex
4-(1-PYRROLIDINYL)BENZONITRILE
Diphenylacetylene
2,4-Dichlorobenzenesulfonyl chloride
METHYL 2,5-DIFLUOROBENZOATE
4-BroMo-3-fluorobenzonitrile
3,5-DibroMo-2-hydroxypyridine, 97%
3-(TrifluoroMethyl)benzaldehyde, 97%
L-Threonine Methyl ester hydrochloride
AntiMony pentoxide
(2S,4S)-(+)-2,4-Pentanediol , 98%
Benzoyleneurea
4-Chloro-3-(ethoxycarbonyl)phenylboronic acid
1-(4-BroMophenyl)-1H-pyrazole
1-Methyl-1H-imidazole-5-carbohydrazide, 97%
4-[(1,1-Dioxidothiomorpholin-4-yl)methyl]benzoic acid
3-Chloro-4-(morpholine-4-carbonyl)benzeneboronic acid 98%
4-Aminophthalic Acid