SL-1细胞系——人胚肺转化细胞系如何消化

SL-1细胞系——人胚肺转化细胞系如何消化 货号：GD-C11219270

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞，全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
3-Nitrobenzaldehyde, 99%
BenzyltriMethylaMMoniuM chloride
Phenethyl phenylacetate
7-METHYL-1,4-BENZOTHIAZIN-3-ONE
D-HoMophenylalanine ethyl ester hydrochloride
1,2:3,4:5,6-tri-O-isopropylidene-D-Mannitol
GiMeracil
(6,7-dihydro-5H-pyrrolo[2,1-c][1,2,4]triazol-3-ylmethyl)amine(SALTDATA: 2.3CF3COOH 0.3H2O)
Potassium (4-formylphenyl)trifluoroborate
4-Chloro-4'-hydroxybenzophenone
(3S,6S)-3-Benzyl-1,4-diazabicyclo[4.3.0]nonane
BOC-4-OXO-PRO-OME
1,2-BENZANTHRACENE
1,4-Dibenzoylbutane
DL-Tartaric acid
6-Chloroindole
Meso-1,2-DiphenylethylenediaMine
Methyl 3-broMo-5-Methoxybenzoate
Methyl 3-isocyanatobenzoate, 97%
Sulfanilic Acid, Anhydrous, Reagent, ACS
N-(3-Hydroxypropyl)ethylenediaMine
4-Isopropylacetophenone
4-Amidinopyridinium chloride
Tetracycline Hydrochloride
2,6-Pyridinedicarboxylic acid
TERBIUM(III) CARBONATE HYDRATE
Ethyl 4,4,4-trifluoroacetoacetate
SaMariuM(Iii) Chloride Hexahydrate
1-Methyl-2-(tri-n-butylstannyl)indole, 96%
Crystal Violet
trichloroacetic acid
Zincon
PotassiuM (4-Methoxycarbonylphenyl)trifluoroborate
O,O'-BIS(TRIMETHYLSILYL)-5-FLUOROURACIL
4-ForMyl-3,5-diMethylphenylboronic acid
2-(2-ChloroacetaMido)-4-thiazoleacetic acid
2-Fluoro-5-(trifluoromethyl)benzylamine
1-[3-(4-Methylpiperazin-1-yl)phenyl]methanamine , 97%
2-Naphthyl Acetate
(4'-Nitro[1,1'-biphenyl]-4-yl)methanol
SEC-BUTYL ISOTHIOCYANATE
2-Phenylbenzimidazole-5-sulfonic acid
FEMA 2871
Isobutyric anhydride
5-BroMo-2-chloropyridine
2,4-Dinitroanisole
Nitrosobenzene
2-Hydrazinyl-6-Methylpyridine
1,2-Dihydronaphthalene, 96%
Potassium hydroxide
2,3-Pyrazinedicarboxylic Acid
4-(tert-butoxycarbonyl)benzoic acid
1-Bromo-4-butylbenzene
poncirin
1-benzyl-3-pyrroline
YTTERBIUM FLUORIDE OXIDE
HEXAMETHONIUM CHLORIDE
4-Fluoro-3-(Morpholine-4-carbonyl)phenylboronic acid
Glyoxal Mono-diMethylhydrazone
1,3-Bis(chloromethyl)tetramethyldisiloxane
5- p -Tolyl-2 H -pyrazole-3-carboxylic acid
Ponceau SX
Methyl (S)-(-)-2-Isocyanato-4-methylvalerate
1-Boc-3-(Amino)azetidine
Bis(trimethylsilyl)trifluoroacetamide
Methyl 3-bromo-4-methylbenzoate
Sulfapyridine Melting Point Standard (ApproxiMately 191 degrees)
Ethyl 4-Piperidinecarboxylate
1-Butoxy-2-propanol
2'-Methoxy-6'-Methylbiphenyl-3-carboxylic acid
6-BroMohexanenitrile, 95%
2-Methyl-6-quinolinecarbaldehyde, 97%
1H,1H,10H,10H-Hexadecafluoro-1,10-decanediol
N,N'-Di-n-octyl-3,4,9,10-perylenetetracarboxylic DiiMide
Furoin
Boc-D-Arg(Mtr)-OH
4-bromo-1-methyl-1H-pyrazole-5-carboxylic acid
N-(3-fluorobenzyl)butan-1-amine
AREG ELISA Kit
Mouse RAPGEF2 (Rap Guanine Nucleotide Exchange Factor 2) ELISA Kit
Human PCDH8 ELISA Kit
Human DPP6 (Dipeptidyl Peptidase VI) ELISA Kit
CD3 ELISA Kit
Aβ1-42 ELISA Kit
PLCγ1 ELISA Kit
Human ELA4 ELISA Kit
Human SAA ELISA Kit
INHβB ELISA Kit
MIO Medium
Modified Y Medium
Copper(II) 2,4-pentanedionate, 98%
Phosphorus(III) fluoride, 99%
(R)-(+)-5,5',6,6',7,7',8,8'-OCTAHYDRO-1,1'-BI-2-NAPHTHOL
2-Fluoro-3-iodopyridine, 97%
4-Isopropylacetophenone
2,5-Dimethylphenylacetic acid
Transferrin
Imidazolidine

2-Bromo-2-nitro-1,3-propanediol
1-Bromo-5-chloropentane
ethyl 1H-indazole-4-carboxylate
HexacarbonylMolybdenuM, 98%
Norbornene, 99%
4-Fluorothioanisole
4-HydroxythiobenzaMide
Isobutyl 1,2-dihydro-2-isobutoxy-1-quinoline-carboxylate
4-(2-MethoxyethylcarbaMoyl)phenylboronic acid, pinacol ester
2-(TRIFLUOROMETHYL)PHENETHYL ALCOHOL
Halosulfuron-Methyl
4-Phenyl-1-buten-4-ol, 97%
N-Boc-2-maleimidoethylamine
5-Hydroxy-1-methyl-3-trifluoromethyl-1H-pyrazole-4-carbaldehyde
Copper(I) bromide-dimethyl sulfide complex, 99%