GFP标签抗体, 抗GFP绿色荧光单抗_详细资料

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Anti GFP Monoclonal Antibody

Calatog Number: LT9994

Lot Number: 411005100210

Isotype: Mouse IgG1

Clonality: GF28R

Quantity: 50 μg

Immunogen: GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates

Specificity: Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP

Purification: Protein A affinity chromatography from mouse ascites fluid

Applications: Dot, ELISA, IP, IS, WB

Sensitivity: WB (with ECL): 1-10ng of purified GFP or GFP fusion proteins

Working Condition: WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

Storage: Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks

Images:
Fig. 1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (1), 1μg (2) and 10μg (3) of cell lysates used

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GFP标签抗体, 抗GFP绿色荧光单抗

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