GAPDH内参抗体,抗GAPDH单抗_详细资料

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Anti GAPDH Monoclonal Antibody

Calatog Number: LT9995

Lot Number: 411005100211

Isotype: Mouse IgG1

Clonality: GA1R

Quantity: 50 μg

Immunogen: Recombinant GAPDH

Specificity: Recognizes native and denatured forms of GAPDH (~37kDa)

Cross Reactivity: GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, chicken, and hamster. GAPDH from other species may also be detectable

Purification: Protein A affinity chromatography from mouse ascites fluid

Applications: Dot, ELISA, IS, WB

Working Condition: WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

Storage: Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks

Images:
Fig. LEFT: 1:2000 (0.5μg/mL) Ab dilution used in WB of 5μg/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5), and hamster (6). RIGHT: WB from BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3)

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