27104 QIAprep Spin Miniprep Kit;Qiagen 27104;原装

产地：Qiagen货号：27104ProcedurePlasmid purification using QIAprep Kits follows a simple bind-wash-elute procedure (see figure "The QIAprep procedure"). First, bacterial cultures are lysed and the lysates are cleared by centrifugation. The cleared lysates are then applied to the QIAprep module where plasmid DNA adsorbs to the silicagel membrane. Impurities are washed away and pure DNA is eluted in a small volume of elution buffer or water. The QIAprep Spin MiniPrep Kit enables purification of up to 20 ug molecular biology grade plasmid DNA or cosmid DNA for use in routine molecular biology applications such as fluorescent and radioactive sequencing and cloning. Versatile QIAprep spin columns can be used either in microcentrifuges or on vacuum manifolds (see figure "QIAprep Spin Column handling options"). The vacuum procedure provides simplified handling and faster sample processing. QIAprep spin columns can be vacuum processed using the QIAvac 24 plus, QIAvac 6S with QIAvac Luer Adapters, or any other commercial manifold with luer connectors. The QIAprep Spin Miniprep Kit can now also be fully automated on the QIAcube. Characteristics   Format Spin columns Purification module QIAprep spin columns  Throughput 1–24 samples  Preparation time 24 minipreps in 30 minutes Equipment required Microcentrifuge or vacuum manifold; fully automatable using the QIAcube  Lysate clearing Centrifugation PrincipleQIAprep spin columns contain a unique silica gel membrane that binds up to 20 µg DNA in the presence of a high concentration of chaotropic salt, and allows elution in a small volume of low-salt buffer. QIAprep membrane technology eliminates time consuming phenol-chloroform extraction and alcohol precipitation, as well as the problems and inconvenience associated with loose resins and slurries. High-purity plasmid DNA eluted from QIAprep spin columns is immediately ready to use - there is no need to precipitate, concentrate, or desalt.