产地:Vectorlabs 货号:MB-6500 规格: 1 KitOne kit contains sufficient reagents to develop approximately twenty 100 cm2 blots. Biotin can be incorporated into a nucleic acid probe using various enzymatic or non-enzymatic methods including the PHOTOPROBE® Biotin, FastTag®, 3'EndTag™, or 5' EndTag™ Labeling Systems. Biotinylated probes are recognized by streptavidin coupled to alkaline phosphatase. The probe is ultimately visualized by the conversion of DuoLuX™ Chemiluminescent/Fluorescent Substrate to a luminescent and fluorescent product by a dephosphorylation reaction catalyzed by alkaline phosphatase. The sensitivity is enhanced with the use of the PolyBlock™ Blocking Reagent and wash buffers included in the kit that are specifically designed to minimize background. The UltraSNAP™ Detection Kit is designed for the detection of biotinylated nucleic acids on nylon or nitrocellulose blots. This kit features the DuoLuX™ Chemiluminescent/Fluorescent Substrate, as well as specially optimized PolyBlock™ Blocking Reagent and washing solutions developed to produce high sensitivity with low background. UltraSNAP™ Kit components include: DuoLuX™ Substrate (100 ml) Alkaline Phosphatase Streptavidin (600 µl) 10x PolyBlock™ Blocking Reagent (120 ml) 20x Wash A (120 ml) 10x Wash B (120 ml)