Biotinylated Human B7-H4, ultra sensitivity (primary amine labeling)

内毒素:
Less than 1.0 EU per μg by the LAL method.

纯度:
>95% as determined by SDS-PAGE.

活性:
Measured by its binding ability in a functional ELISA against immobilized anti-B7-H4 mAb (Human IgG1, 0.2 μg/well). The linear range is 4-62 ng/mL.

产品背景:
V-set domain-containing T-cell activation inhibitor 1 (VTCN1) is also known as Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, B7H4, which belongs to the immunoglobulin superfamily and BTN/MOG family. VTCN1 contains two Ig-like V-type (immunoglobulin-like) domains. The expression of VTCN1 is up-regulated by IL6 and IL10 and is inhibited by GM-CSF and IL4 on antigen-presenting cells (APCs). VTCN1 / B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. VTCN1 involved in promoting epithelial cell transformation.

产品描述:
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.1 kDa. The protein migrates as 43-60 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.