Biotinylated Human TFPI, ultra sensitivity (primary amine labeling)

内毒素:
Less than 1.0 EU per μg by the LAL method.

纯度:
>90% as determined by SDS-PAGE.

活性:


产品背景:
Tissue factor pathway inhibitor (TFPI) is also known as Extrinsic pathway inhibitor (EPI), Lipoprotein - associated coagulation inhibitor (LACI), is a plasma proteinase inhibitor synthesized by vascular endothelial cells and part of it is associated with glycosaminoglycans of these cells. TFPI is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). TFPI is a secreted protein with a N­terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C­terminal basic region. The first K domain inhibits coagulation factor VIIa complexed to tissue factor (TF); The second K domain inhibits factor Xa; The third K domain binds to heparin; The C­terminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces.

产品描述:
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 30 kDa. The protein migrates as 41-45 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.