Biotinylated Human Her2 / ErbB2, ultra sensitivity (primary amine labeling, long spacer)

内毒素:
Less than 1.0 EU per μg by the LAL method.

纯度:
>95% as determined by reduced SDS-PAGE.

活性:
Measured by its binding ability in a functional ELISA against immobilized trastuzumab (0.3 μg/well). The EC50 is 0.09-1 ng/mL.

产品背景:
Human Epidermal growth factor Receptor 2 (HER2),also called ERBB2, HER-2,HER-2/neu, NEU, NGL,TKR1 and c-erb B2,and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30% of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer,communication at the neuromuscular junction andregulation of cell growth and differentiation.

产品描述:
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 70.2 kDa. The protein migrates as 110-115 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.