High Pure Plasmid Isolation Kit
packaging | pkg of 250 purifications (11754785001) |
| pkg of 50 purifications (11754777001) |
产品描述
Components
• Suspension Buffer
• RNase A, dry powder
• Lysis Buffer
• Binding Buffer
• Wash Buffer I
• Wash Buffer II
• Elution Buffer
• High Pure Spin Filter Tubes (containing glass fiber fleece)
• Collection Tubes
The kit relies on alkaline lysis to release plasmid DNA from bacteria. RNase removes all RNA in the lysate. After cellular debris and (entrapped) genomic DNA are removed by centrifugation, the remaining supernatant is mixed with a chaotropic salt and applied to the glass fiber fleece in a High Pure Spin Filter Tube. Under the buffer conditions used in the procedure, the plasmid binds to the glass fiber fleece, while contaminating substances (salts, proteins, and other cellular contaminants) do not. Brief wash-and-spin steps readily remove these contaminants. Once purified, the plasmid can be easily eluted in a small volume of low-salt buffer or water.
Roche试剂
11754785001 | High Pure Plasmid Isolation Kit HP质粒提取试剂盒 |
10102121001 | Adenosine Deaminase (ADA), sus, 腺苷脱氨酶 |
10691836001 | Glycerokinase, 甘油激酶 |
11119915001 | RNase, DNase-free, 不含水,RNA酶和DNA酶 |
11775367001 | Uracil-DNA Glycosylase,Heat-la, 尿嘧啶- DNA糖基化酶 |
10105589001 | GPT, 10 mg, 谷丙转氨酶 |
10621722001 | Creatine phosphate, 10g, 磷酸肌酸 |
12011875001 | HP Viral Nucleic Acid Buffer S, HP病毒核酸缓冲S |
11775375001 | Uracil-DNA Glycosylase,Heat-la, 尿嘧啶- DNA糖基化酶 |
11785834001 | ExpandTM Reverse Transcriptase, 逆转录酶 |
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温馨提示:不可用于临床ZL。