【产品介绍】DNA-activated serine/threonine protein kinase (DNA-PK) is a nuclear protein kinase that is involved in DNA nonhomologous end joining (NHEJ) required for double- strand brake repair and V(D)J recombination. DNA-PK is composed of a large catalytic subunit of ~470 kDa (DNA-PKcs) and a DNA binding heterodimer of approximately 70- and 80-kDa subunits called Ku. (1-2). Autophosphorylation of DNA-PK correlates with loss of protein kinase activity and dissociation of the DNA-PKcs-Ku complex. DNA-PK phosphorylates Hsp90, p53 and c-Jun (3-4). DNA-PK/PRKDC Phospho (pS2056),Rabbit Recommended Applications WB, IHC Applications and Recommended Dilution Factors WB: 1:1,000 - 10,000 IHC: 1:100 - 250 Species Reactivity * DNA-PK/PRKDC Phospho (pS2056),Rabbit Human *Species cross-reactivity is based on WB analysis Products Data Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-Phospho-DNA-PK/PRKDC (pS2056) RabMAb (cat. #3892-1). Western blot analysis on Jurkat cell lysates using anti-Phospho-DNA-PK/PRKDC (pS2056) RabMAb (cat. #3892-1). Cells were either (A) untreated (B) treated with Alkaline Phosphatase. DNA-PK/PRKDC Phospho (pS2056),Rabbit Specificity A phospho specific peptide corresponding to residues surrounding Serine 2056 of human DNA-PK/PRKDC was used as an immunogen. This antibody only detects DNA-PK/PRKDC phosphorylated at Serine 2056. |