pCDNA-dcas9 p300core(Plasmid #61357) CRISPR/CAS9 质粒 载体

测序及更多信息：https://pan.baidu.com/s/1pFFXnsLoIyILKegXmrP9rA 密码：b3zo

采购地址：淘宝店铺地址

载体作用

encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

载体骨架

pcDNA3.1

构建方法

载体类型

哺乳动物表达， CRISPR

筛选标记

Neomycin (select with G418)

插入基因信息

S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300)

参考文献

Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy TE, Gersbach CA. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6. 10.1038/nbt.3199 PubMed 25849900

载体图谱↑蓝色为测序比对区

全序列

1        GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG

61       CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG

121      CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC

181      TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT

241      GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA

301      TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC

361      CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC

421      ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT

481      ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT

541      ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA

601      TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG

661      ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC

721      AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG

781      GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA

841      CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC

901      GCCATGGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA TTACAAGGAT

961      GACGATGACA AGATGGCCCC CAAGAAGAAG AGGAAGGTGG GCCGCGGAAT GGATAAGAAA

1021     TACTCAATAG GCTTAGCTAT CGGCACAAAT AGCGTCGGAT GGGCGGTGAT CACTGATGAA

1081     TATAAGGTTC CGTCTAAAAA GTTCAAGGTT CTGGGAAATA CAGACCGCCA CAGTATCAAA

1141     AAAAATCTTA TAGGGGCTCT TTTATTTGAC AGTGGAGAGA CAGCGGAAGC GACTCGTCTC

1201     AAACGGACAG CTCGTAGAAG GTATACACGT CGGAAGAATC GTATTTGTTA TCTACAGGAG

1261     ATTTTTTCAA ATGAGATGGC GAAAGTAGAT GATAGTTTCT TTCATCGACT TGAAGAGTCT

1321     TTTTTGGTGG AAGAAGACAA GAAGCATGAA CGTCATCCTA TTTTTGGAAA TATAGTAGAT

1381     GAAGTTGCTT ATCATGAGAA ATATCCAACT ATCTATCATC TGCGAAAAAA ATTGGTAGAT

1441     TCTACTGATA AAGCGGATTT GCGCTTAATC TATTTGGCCT TAGCGCATAT GATTAAGTTT

1501     CGTGGTCATT TTTTGATTGA GGGAGATTTA AATCCTGATA ATAGTGATGT GGACAAACTA

1561     TTTATCCAGT TGGTACAAAC CTACAATCAA TTATTTGAAG AAAACCCTAT TAACGCAAGT

1621     GGAGTAGATG CTAAAGCGAT TCTTTCTGCA CGATTGAGTA AATCAAGACG ATTAGAAAAT

1681     CTCATTGCTC AGCTCCCCGG TGAGAAGAAA AATGGCTTAT TTGGGAATCT CATTGCTTTG

1741     TCATTGGGTT TGACCCCTAA TTTTAAATCA AATTTTGATT TGGCAGAAGA TGCTAAATTA

1801     CAGCTTTCAA AAGATACTTA CGATGATGAT TTAGATAATT TATTGGCGCA AATTGGAGAT

1861     CAATATGCTG ATTTGTTTTT GGCAGCTAAG AATTTATCAG ATGCTATTTT ACTTTCAGAT

1921     ATCCTAAGAG TAAATACTGA AATAACTAAG GCTCCCCTAT CAGCTTCAAT GATTAAACGC

1981     TACGATGAAC ATCATCAAGA CTTGACTCTT TTAAAAGCTT TAGTTCGACA ACAACTTCCA

2041     GAAAAGTATA AAGAAATCTT TTTTGATCAA TCAAAAAACG GATATGCAGG TTATATTGAT

2101     GGGGGAGCTA GCCAAGAAGA ATTTTATAAA TTTATCAAAC CAATTTTAGA AAAAATGGAT

2161     GGTACTGAGG AATTATTGGT GAAACTAAAT CGTGAAGATT TGCTGCGCAA GCAACGGACC

2221     TTTGACAACG GCTCTATTCC CCATCAAATT CACTTGGGTG AGCTGCATGC TATTTTGAGA

2281     AGACAAGAAG ACTTTTATCC ATTTTTAAAA GACAATCGTG AGAAGATTGA AAAAATCTTG

2341     ACTTTTCGAA TTCCTTATTA TGTTGGTCCA TTGGCGCGTG GCAATAGTCG TTTTGCATGG

2401     ATGACTCGGA AGTCTGAAGA AACAATTACC CCATGGAATT TTGAAGAAGT TGTCGATAAA

2461     GGTGCTTCAG CTCAATCATT TATTGAACGC ATGACAAACT TTGATAAAAA TCTTCCAAAT

2521     GAAAAAGTAC TACCAAAACA TAGTTTGCTT TATGAGTATT TTACGGTTTA TAACGAATTG

2581     ACAAAGGTCA AATATGTTAC TGAAGGAATG CGAAAACCAG CATTTCTTTC AGGTGAACAG

2641     AAGAAAGCCA TTGTTGATTT ACTCTTCAAA ACAAATCGAA AAGTAACCGT TAAGCAATTA

2701     AAAGAAGATT ATTTCAAAAA AATAGAATGT TTTGATAGTG TTGAAATTTC AGGAGTTGAA

2761     GATAGATTTA ATGCTTCATT AGGTACCTAC CATGATTTGC TAAAAATTAT TAAAGATAAA

2821     GATTTTTTGG ATAATGAAGA AAATGAAGAT ATCTTAGAGG ATATTGTTTT AACATTGACC

2881     TTATTTGAAG ATAGGGAGAT GATTGAGGAA AGACTTAAAA CATATGCTCA CCTCTTTGAT

2941     GATAAGGTGA TGAAACAGCT TAAACGTCGC CGTTATACTG GTTGGGGACG TTTGTCTCGA

3001     AAATTGATTA ATGGTATTAG GGATAAGCAA TCTGGCAAAA CAATATTAGA TTTTTTGAAA

3061     TCAGATGGTT TTGCCAATCG CAATTTTATG CAGCTGATCC ATGATGATAG TTTGACATTT

3121     AAAGAAGACA TTCAAAAAGC ACAAGTGTCT GGACAAGGCG ATAGTTTACA TGAACATATT

3181     GCAAATTTAG CTGGTAGCCC TGCTATTAAA AAAGGTATTT TACAGACTGT AAAAGTTGTT

3241     GATGAATTGG TCAAAGTAAT GGGGCGGCAT AAGCCAGAAA ATATCGTTAT TGAAATGGCA

3301     CGTGAAAATC AGACAACTCA AAAGGGCCAG AAAAATTCGC GAGAGCGTAT GAAACGAATC

3361     GAAGAAGGTA TCAAAGAATT AGGAAGTCAG ATTCTTAAAG AGCATCCTGT TGAAAATACT

3421     CAATTGCAAA ATGAAAAGCT CTATCTCTAT TATCTCCAAA ATGGAAGAGA CATGTATGTG

3481     GACCAAGAAT TAGATATTAA TCGTTTAAGT GATTATGATG TCGATGCCAT TGTTCCACAA

3541     AGTTTCCTTA AAGACGATTC AATAGACAAT AAGGTCTTAA CGCGTTCTGA TAAAAATCGT

3601     GGTAAATCGG ATAACGTTCC AAGTGAAGAA GTAGTCAAAA AGATGAAAAA CTATTGGAGA

3661     CAACTTCTAA ACGCCAAGTT AATCACTCAA CGTAAGTTTG ATAATTTAAC GAAAGCTGAA

3721     CGTGGAGGTT TGAGTGAACT TGATAAAGCT GGTTTTATCA AACGCCAATT GGTTGAAACT

3781     CGCCAAATCA CTAAGCATGT GGCACAAATT TTGGATAGTC GCATGAATAC TAAATACGAT

3841     GAAAATGATA AACTTATTCG AGAGGTTAAA GTGATTACCT TAAAATCTAA ATTAGTTTCT

3901     GACTTCCGAA AAGATTTCCA ATTCTATAAA GTACGTGAGA TTAACAATTA CCATCATGCC

3961     CATGATGCGT ATCTAAATGC CGTCGTTGGA ACTGCTTTGA TTAAGAAATA TCCAAAACTT

4021     GAATCGGAGT TTGTCTATGG TGATTATAAA GTTTATGATG TTCGTAAAAT GATTGCTAAG

4081     TCTGAGCAAG AAATAGGCAA AGCAACCGCA AAATATTTCT TTTACTCTAA TATCATGAAC

4141     TTCTTCAAAA CAGAAATTAC ACTTGCAAAT GGAGAGATTC GCAAACGCCC TCTAATCGAA

4201     ACTAATGGGG AAACTGGAGA AATTGTCTGG GATAAAGGGC GAGATTTTGC CACAGTGCGC

4261     AAAGTATTGT CCATGCCCCA AGTCAATATT GTCAAGAAAA CAGAAGTACA GACAGGCGGA

4321     TTCTCCAAGG AGTCAATTTT ACCAAAAAGA AATTCGGACA AGCTTATTGC TCGTAAAAAA

4381     GACTGGGATC CAAAAAAATA TGGTGGTTTT GATAGTCCAA CGGTAGCTTA TTCAGTCCTA

4441     GTGGTTGCTA AGGTGGAAAA AGGGAAATCG AAGAAGTTAA AATCCGTTAA AGAGTTACTA

4501     GGGATCACAA TTATGGAAAG AAGTTCCTTT GAAAAAAATC CGATTGACTT TTTAGAAGCT

4561     AAAGGATATA AGGAAGTTAA AAAAGACTTA ATCATTAAAC TACCTAAATA TAGTCTTTTT

4621     GAGTTAGAAA ACGGTCGTAA ACGGATGCTG GCTAGTGCCG GAGAATTACA AAAAGGAAAT

4681     GAGCTGGCTC TGCCAAGCAA ATATGTGAAT TTTTTATATT TAGCTAGTCA TTATGAAAAG

4741     TTGAAGGGTA GTCCAGAAGA TAACGAACAA AAACAATTGT TTGTGGAGCA GCATAAGCAT

4801     TATTTAGATG AGATTATTGA GCAAATCAGT GAATTTTCTA AGCGTGTTAT TTTAGCAGAT

4861     GCCAATTTAG ATAAAGTTCT TAGTGCATAT AACAAACATA GAGACAAACC AATACGTGAA

4921     CAAGCAGAAA ATATTATTCA TTTATTTACG TTGACGAATC TTGGAGCTCC CGCTGCTTTT

4981     AAATATTTTG ATACAACAAT TGATCGTAAA CGATATACGT CTACAAAAGA AGTTTTAGAT

5041     GCCACTCTTA TCCATCAATC CATCACTGGT CTTTATGAAA CACGCATTGA TTTGAGTCAG

5101     CTAGGAGGTG ACCCAATTGC CGGATCCAAG GCTAGCCCGA AAAAGAAACG CAAAGTTGGG

5161     CGCGCCATTT TCAAACCAGA AGAACTACGA CAGGCACTGA TGCCAACTTT GGAGGCACTT

5221     TACCGTCAGG ATCCAGAATC CCTTCCCTTT CGTCAACCTG TGGACCCTCA GCTTTTAGGA

5281     ATCCCTGATT ACTTTGATAT TGTGAAGAGC CCCATGGATC TTTCTACCAT TAAGAGGAAG

5341     TTAGACACTG GACAGTATCA GGAGCCCTGG CAGTATGTCG ATGATATTTG GCTTATGTTC

5401     AATAATGCCT GGTTATATAA CCGGAAAACA TCACGGGTAT ACAAATACTG CTCCAAGCTC

5461     TCTGAGGTCT TTGAACAAGA AATTGACCCA GTGATGCAAA GCCTTGGATA CTGTTGTGGC

5521     AGAAAGTTGG AGTTCTCTCC ACAGACACTG TGTTGCTACG GCAAACAGTT GTGCACAATA

5581     CCTCGTGATG CCACTTATTA CAGTTACCAG AACAGGTATC ATTTCTGTGA GAAGTGTTTC

5641     AATGAGATCC AAGGGGAGAG CGTTTCTTTG GGGGATGACC CTTCCCAGCC TCAAACTACA

5701     ATAAATAAAG AACAATTTTC CAAGAGAAAA AATGACACAC TGGATCCTGA ACTGTTTGTT

5761     GAATGTACAG AGTGCGGAAG AAAGATGCAT CAGATCTGTG TCCTTCACCA TGAGATCATC

5821     TGGCCTGCTG GATTCGTCTG TGATGGCTGT TTAAAGAAAA GTGCACGAAC TAGGAAAGAA

5881     AATAAGTTTT CTGCTAAAAG GTTGCCATCT ACCAGACTTG GCACCTTTCT AGAGAATCGT

5941     GTGAATGACT TTCTGAGGCG ACAGAATCAC CCTGAGTCAG GAGAGGTCAC TGTTAGAGTA

6001     GTTCATGCTT CTGACAAAAC CGTGGAAGTA AAACCAGGCA TGAAAGCAAG GTTTGTGGAC

6061     AGTGGAGAGA TGGCAGAATC CTTTCCATAC CGAACCAAAG CCCTCTTTGC CTTTGAAGAA

6121     ATTGATGGTG TTGACCTGTG CTTCTTTGGC ATGCATGTTC AAGAGTATGG CTCTGACTGC

6181     CCTCCACCCA ACCAGAGGAG AGTATACATA TCTTACCTCG ATAGTGTTCA TTTCTTCCGT

6241     CCTAAATGCT TGAGGACTGC AGTCTATCAT GAAATCCTAA TTGGATATTT AGAATATGTC

6301     AAGAAATTAG GTTACACAAC AGGGCATATT TGGGCATGTC CACCAAGTGA GGGAGATGAT

6361     TATATCTTCC ATTGCCATCC TCCTGACCAG AAGATACCCA AGCCCAAGCG ACTGCAGGAA

6421     TGGTACAAAA AAATGCTTGA CAAGGCTGTA TCAGAGCGTA TTGTCCATGA CTACAAGGAT

6481     ATTTTTAAAC AAGCTACTGA AGATAGATTA ACAAGTGCAA AGGAATTGCC TTATTTCGAG

6541     GGTGATTTCT GGCCCAATGT TCTGGAAGAA AGCATTAAGG AACTGGAACA GGAGGAAGAA

6601     GAGAGAAAAC GAGAGGAAAA CACCAGCAAT GAAAGCACAG ATGTGACCAA GGGAGACAGC

6661     AAAAATGCTA AAAAGAAGAA TAATAAGAAA ACCAGCAAAA ATAAGAGCAG CCTGAGTAGG

6721     GGCAACAAGA AGAAACCCGG GATGCCCAAT GTATCTAACG ACCTCTCACA GAAACTATAT

6781     GCCACCATGG AGAAGCATAA AGAGGTCTTC TTTGTGATCC GCCTCATTGC TGGCCCTGCT

6841     GCCAACTCCC TGCCTCCCAT TGTTGATCCT GATCCTCTCA TCCCCTGCGA TCTGATGGAT

6901     GGTCGGGATG CGTTTCTCAC GCTGGCAAGG GACAAGCACC TGGAGTTCTC TTCACTCCGA

6961     AGAGCCCAGT GGTCCACCAT GTGCATGCTG GTGGAGCTGC ACACGCAGAG CCAGGACTAC

7021     CCGTACGACG TTCCGGACTA CGCTTCTTGA GTTTAAACCG CTGATCAGCC TCGACTGTGC

7081     CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG

7141     GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA

7201     GGTGTCATTC TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG

7261     ACAATAGCAG GCATGCTGGG GATGCGGTGG GCTCTATGGC TTCTGAGGCG GAAAGAACCA

7321     GCTGGGGCTC TAGGGGGTAT CCCCACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG

7381     TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG

7441     CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG

7501     GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT

7561     AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT

7621     TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA

7681     TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA

7741     ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTAATTCTG TGGAATGTGT GTCAGTTAGG

7801     GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA

7861     GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT

7921     GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC

7981     TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA

8041     GGCCGAGGCC GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG

8101     CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA

8161     GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC

8221     CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA

8281     TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT

8341     GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC

8401     GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT

8461     ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT

8521     ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT

8581     CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT

8641     CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG

8701     GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT

8761     GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG

8821     TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG

8881     CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG

8941     CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG

9001     ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT

9061     GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG

9121     GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC

9181     AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT

9241     TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC

9301     TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA

9361     ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG

9421     AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG

9481     TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC

9541     TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA

9601     TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG

9661     AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG

9721     TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG

9781     TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG

9841     CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA

9901     AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC

9961     TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT

10021    AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT

10081    GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG

10141    CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT

10201    ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT

10261    TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG

10321    ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC

10381    ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA

10441    TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG

10501    GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG

10561    TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA

10621    GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG

10681    CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA

10741    GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC

10801    ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA

10861    AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG

10921    ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT

10981    AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC

11041    AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG

11101    GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG

11161    GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT

11221    GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA

11281    GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA

11341    CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC

11401    ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA

11461    GTGCCACCTG ACGTC