16181 QIAGEN Plasmid Plus 96 Miniprep Kit;Qiagen 16181

产地：Qiagen货号：16181Principle and procedureQIAGEN Plasmid Plus 96 Miniprep and BioRobot Kits are based on QIAGEN Plasmid Plus technology. This novel method for plasmid preparation in 96-well format provides highly pure, Transfection Grade plasmid DNA using a simple bind-wash-elute procedure (see figure "QIAGEN Plasmid Plus 96 procedure"). After neutralization, lysates are cleared using a TurboFilter 96 plate. A nonchaotropic binding buffer is added to the cleared lysate to optimize plasmid DNA binding to the membrane of the Plasmid Plus 96 plate. The unique binding buffer provides very specific binding conditions leading to DNA quality that is comparable to anion-exchange preps. A wash step with an endotoxin reduction buffer results in Transfection Grade plasmid DNA suited for transfection into most cell lines (see figure "Highly efficient transfection into a sensitive cell lines"). The proprietary binding chemistry allows purification of up to 50 μg plasmid DNA per well from a 5 ml E. coli culture (see figure "High yields of up to 50 µg plasmid DNA achieved using the QIAGEN Plasmid Plus 96 Miniprep Kit"). Convenient and flexible processing options The procedure may be automated using the QIAGEN Plasmid Plus 96 BioRobot Kit on the BioRobot Universal System. The QIAvac 96 is used for lysate clearing using the TurboFilter 96 plate, and DNA binding and wash steps are performed using the Plasmid Plus 96 plate. Drying and elution steps may either be performed on the QIAvac 96 or using a compatible centrifuge. Alternatively, the complete procedure can be performed using a compatible centrifuge. Very low endotoxin levels Kits include an endotoxin reduction buffer that effectively reduces bacterial endotoxins to levels typically lower than 1 EU/μg, providing plasmid DNA quality which is suitable for transfection into sensitive cell lines (see figures "Very low endotoxin levels in plasmid DNA" and "Highly efficient transfection into a sensitive cell line"). Consistently high yields of ready-to-use plasmid DNA QIAGEN Plasmid Plus technology delivers the same performance and quality as anion-exchange technology. High yields of up to 50 μg of Transfection Grade plasmid DNA can be obtained (see figure ""High yields of up to 50 µg plasmid DNA achieved using the QIAGEN Plasmid Plus 96 Miniprep Kit").