| Anti Anti-DYKDDDDK(FLAG) Monoclonal Antibody | 说明书下载 | Calatog Number: | LT0420 | Lot Number: | 411005100203 | Isotype: | Mouse IgG2b | Clonality: | FG4R | Quantity: | 50 μg | Immunogen: | DYKDDDDK (FLAG) synthetic peptide conjugated to KLH | Specificity: | Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins | Purification: | Protein A affinity chromatography from mouse ascites fluid | Applications: | Dot, ELISA, IS, IP, WB | Sensitivity: | WB (with ECL): 1-5ng of DYKDDDDK-tagged fusion proteins | Working Condition: | WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test | Storage: | Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks | Images: | Fig. 1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector; untransfected (1) and transfected (2) | |