性 状: | 【反应特异性】Human , Mouse Akt1/PKBa Phospho (pS473),Rabbit Monocl 【克隆 ID】EP2109Y 【用途】WB , IHC , ICC 【抗原分子量】60 kDa |
质量标准: | 【产品介绍】Akt1/PKBa is a Ser/Thr protein kinase whose kinase activity plays a key role in various cellular functions, including apoptosis, glycogen synthesis, a n d cell growth . Akt1/PKBa is activated by various growth a n d survival fact o r s (1). Phosph o r ylation on Thr308, Ser473 a n d Tyr474 is required f o r full activity. Akt1/PKBa inhibits apoptosis by phosph o r ylating a n d inactivating several targets, including caspase-9 (2). Akt1/PKBa regulates glycogen synthesis by phosph o r ylating a n d inactivating glycogen synthase kinase-3a a n d b (3,4). Three mammalian Akt1/PKBa isof o r ms have been identified. Akt1/PKBa Phospho (pS473),Rabbit Monocl WB: 1:5000 - 10,000 IHC: 1:100 250 ICC: 1:100 250 Akt1/PKBa Phospho (pS473),Rabbit Monocl A. Immunohistochemical analysis of untreated cervical carcinoma using anti-Phospho-Akt1 (pS473) RabMAb (cat. # 2118-1). B. Immunohistochemical analysis of Phosphatase- treated cervical carcinoma using anti-Phospho-Akt1 (pS473) RabMAb (cat. # 2118-1). C. Western blot analysis on 3T3 cell lysates Akt1/PKBa Phospho (pS473),Rabbit Monocl using anti-Phospho-Akt1 (pS473) RabMAb (cat. #2118-1), 1:10,000 dilution. Cells were either (A) untreated o r (B) treated with PDGF. |