ActiveMax® Recombinant Mouse VEGF164 (HPLC-verified)

内毒素:
Less than 1.0 EU per μg by the LAL method.

纯度:
>95% as determined by SDS-PAGE.

活性:
Measured by its binding ability in a functional ELISA.Immobilized ActiveMax® Mouse VEGF164 (Cat# VE4-M4216) at 2 μg/mL (100 μL/well) can bind VEGFR2 / R3-Fc with a linear range of 0.3-2.5 ng/mL.

产品背景:
Vascular endothelial growth factor A (VEGFA) is also known as Vascular permeability factor (VPF). VEGFA belongs to the PDGF/VEGF growth factor family. VEGFA is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. VEGFA is produced by a group of three major isoforms as a result of alternative splicing and if any three isoforms are produced (VEGFA120, VEGFA164, and VEGFA188) then this will not result in vessel defects and death of the full VEGFA knockout in mice.

产品描述:
ActiveMax® Mouse VEGF164 contains no "tag", and has a calculated MW of 19.3 kDa. The predicted N-terminus is Ala 27. The reducing (R) protein migrates as 23-25 kDa in SDS-PAGE due to different glycosylation.