ActiveMax® Recombinant Human TNF-alpha (HPLC-verified)

内毒素:
Less than 0.1 EU per μg by the LAL method.

纯度:
>95% as determined by SDS-PAGE.

活性:
Measured by its binding ability in a functional ELISA. Immobilized ActiveMax® Human TNF-alpha (Catalog # TNA-H4211) at 5 μg/mL ( 100 μl/well ) can bind Humira with a linear range of 0.04-0.6 ng/mL.

产品背景:
Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.

产品描述:
ActiveMax® Human TNF-alpha (HPLC-verified) contains no "tag", and has a calculated MW of 17.4 kDa. The predicted N-terminus is Val 77. The reducing (R) protein migrates as 18 kDa in SDS-PAGE.