ActiveMax® Recombinant Human VEGF110 (HPLC-verified)

内毒素:
Less than 1.0 EU per μg by the LAL method.

纯度:
>95% as determined by SDS-PAGE.

活性:
(1) Measured by its binding ability in a functional ELISA.Immobilized ActiveMax® Human VEGF110 (Cat# VE0-H5212) at 2 μg/mL (100 μL/well) can bind VEGFR2 / R3-Fc with a linear range of 0.15-5 ng/mL.(2) Measured by its binding ability in a functional ELISA.Immobilized ActiveMax® Human VEGF110 (Cat# VE0-H5212) at 2 μg/mL (100 μL/well) can bind Human VEGF R1 Protein, His Tag (Cat# VE1-H5220) with a linear range of 3-100 ng/mL.

产品背景:
Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, and is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. Alternatively spliced isoforms of 110,121,145,165,183,189 and 206 amino acids in length are expressed in humans.

产品描述:
ActiveMax® Human VEGF110 contains no "tag", and has a calculated MW of 12.6 kDa. The predicted N-terminus is Ala 27. As a result of glycosylation, the reducing (R) protein migrates as 18-20 kDa, and the non-reducing (NR) protein migrates as 40-45 kDa (homodimer) in SDS-PAGE.