Description | | Avidin/Biotin Blocking System (Formerly Signet Catalog No.: 1126) |
Intended Use | | **Research Use Only (RUO)** This product is sold for laboratory research use only, not for human or in-vivo use. |
Form | | Avidin/Biotin Blocking System (SIGNET)Blocking Kit |
Specificity | | Signets Avidin/Biotin Blocking System is intended for use in immunohistochemistry and immunoblotting procedures. The purpose is to decrease the signal caused by endogenous avidin, biotin, or biotin-binding proteins when immunostaining specimens with detection systems that rely on avidin-biotin or steptavidin-biotin chemistry. Nonspecific staining due to these factors commonly resembles a diffuse, weak, cytoplasmic pattern. Kit Contains: - Avidin Solution, 15 mL
- Biotin Solution, 15mL
Both reagents are provided ready-to-use and need no dilution |
Suggested Working Dilution | | The optimal working dilution should be determined for each specific assay condition. Blocking Procedure: Avidin/Biotin Blocking System (SIGNET)For paraffin-embedded tissues: (To be performed before primary antibody incubation and after antigen retrieval). 1. Incubate the Avidin Solution for 10-15 minutes at RT. 2. Rinse with PBS or TBS buffer 3-4 times. 3. Incubate with the Biotin Solution for 10-15 minutes at RT. 4. Rinse with PBS or TBS Buffer 3-4 times. |
Storage | | Store between 2-8°C. |
Warranty/Conditions | | Covance products may not be resold or modified for resale without prior written approval. |