【产品介绍】DNA-activated serine/threonine protein kinase (DNA-PK) is a nuclear protein kinase that is involved in DNA nonhomologous end joining (NHEJ) required for double- strand brake repair and V(D)J recombination. DNA-PK is composed of a large catalytic subunit of ~470 kDa (DNA-PKcs) and a DNA binding heterodimer of approximately 70- and 80-kDa subunits called Ku. (1-2). Autophosphorylation of DNA-PK correlates with loss of protein kinase activity and dissociation of the DNA-PKcs-Ku complex. DNA-PK phosphorylates Hsp90, p53 and c-Jun (3-4). DNA-PK / PRKDC,Rabbit Monoclonal Antibod Recommended Applications WB, ICC Applications and Recommended Dilution Factors WB: 1:1,000 - 10,000 ICC: 1:500 - 1000 Species Reactivity * Human *Species cross-reactivity is based on WB analysis DNA-PK / PRKDC,Rabbit Monoclonal Antibod Products Data Western blot analysis on (A) K562, (B) Molt-4, (C) MCF-7, (D) SH-SY5Y, (E) 293T, and (F) PC-3 cell lysates using anti-DNA-PK / PRKDC RabMAb (cat. #3922-1). Specificity A synthetic peptide corresponding to residues in human DNA-PK / PRKDC was used as an immunogen. |