【产品介绍】eIF-3B is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP: methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (1). eIF-3B (EIF3B),Rabbit Monoclonal Antibod Recommended Applications WB, ICC, IP, FC Applications and Recommended Dilution Factors WB: 1:10,000 - 50,000 ICC: 1:100 - 250 IP: 1:10 - 100 FC: 1:10 - 100 Species Reactivity * Human, Mouse, Rat eIF-3B (EIF3B),Rabbit Monoclonal Antibod *Species cross-reactivity is based on WB analysis. Products Data Western blot analysis on (A) A431, (B) 293T, (C) Jurkat, (D) HeLa, and (E) Caco-2 cell lysates using anti-eIF-3B RabMAb (cat. #5423-1). eIF-3B (EIF3B),Rabbit Monoclonal Antibod Flow cytometric analysis of permeabilized HeLa cells using anti-eIF-3B RabMAb (red) (catalog # 5423-1) or a rabbit IgG (negative) (green). Specificity A synthetic peptide corresponding to residues in human eIF-3B was used as an immunogen. |